Semen Samples Semen samples were obtained from males attending infertility clinic at Government Multispeciality Hospital, Sector 16, Chandigarh, by masturbation into sterile wide mouth container. Applying the statistical mathematical formula to TEM data [ 14 ], FI, an indicator of sperm quality, was determined together with the percentage of sperm pathologies such as immaturity, apoptosis and necrosis. Asian J Androl ;9: How to cite this article: Arch Ital Urol Androl. The bacteria responsible for semen contaminations generally originate from the urinary tract of patients or can be transmitted by the partner via sexual intercourse [ 5 ].
Aetiology and pathogenesis of benign prostatic hyperplasia. RESULTS We assessed consecutive men referred for symptoms consistent with chronic prostatitis, but only 70 of them completed all the tests and were considered in the study. Spermioculture in the patients yielded E. The prevalent opinion is that leukocytospermia is always concomitant with bacteriospermia; however the lack of leukocytospermia does not preclude the development of genitourinary symptoms of disease [ 20 ]. Establishing the timing of the samples so that the Meares and Stamey test always followed the alternative test prevented contamination of the samples for the alternative test from expressed prostatic secretions. Dose-and time-dependent in vitro effects of quercetin on bovine spermatozoa activity and superoxide production. PCR-based technique for the detection of bacteria in semen and urine.
Semen and urine culture in the diagnosis of chronic bacterial prostatitis
Effect of endotoxin-induced reactive oxygen species on sperm motility. Sperm washing and swim-up technique using antibiotics removes microbes from human semen. Molecular Reproduction and Development, A wet mount was prepared and observed under fluorescent microscope x magnification. Reactive oxygen species and sperm DNA damage in infertile men presenting with low level leukocytospermia. Detection of bacteria from semen of infertile males and their seminal parameters.
Journal of Urology, Influence of urogenital infections on sperm functions. It is not clear, yet, whether the presence of anaerobes in the semen may influence fertility. Further characterization was done using LC-MS. BMC Infect Dis ;7: The possible mechanisms of sperm damage, in addition to adhesion by flagella and pili, include the production of toxins and metabolic products originating from bacterial proliferation. Human spermatozoa may therefore share epitopes with antigens of the most frequent species colonizing the genitourinary tract of man.